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and Uhlenbeck OC (1987) Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA template
Cat#ON-268/ON-268-G
heat the tube to 37℃ for 5 minutes and mix thoroughly to resuspend the precipitate
[1] Manoharan
mRNA Cap-2'-O-Methyltransferase RNases and inhibitors and Uhlenbeck OC (1987) OligoribonucleotideProduct Information mRNA Cap 2' O Methyltransferase is a recombinant protein from Vaccinia virus. The enzyme can add a methyl group at the 2' O position of the first nucleotide adjacent to the Cap structure at the 5' end of the RNA. The enzyme utilizes SAM as a methyl donor to methylate capped RNA (Cap 0) resulting in a cap 1 structure. The Cap 1 structure has been reported to enhance mRNA translation efficiency and hence may help improve expression
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