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add 250 &mu to each well of a 24-well plate
2 Primary antibody incubation
liver cirrhosis)
lay the plate
Human Cath-L ELISA Kit Size:96T add 250 &mu to eachProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20