Human IL-29 ELISA Kit RNA Pull Down ASM/H12 Immunogen Synthetic Peptide Location

ASM/H12 Immunogen Synthetic Peptide Location Cytoplasm

Fibroblast growth factor 12 (FGF-12)

checked by color reaction OD Low value Incorrect plate reader settings Check instrument wavelength No stop solution added Add an appropriate amount of stop solution Wait time too long when reading the board Timely plate reading Excessive sample content The appropriate dilution factor was determined by pre-experiment Sample content is too low The appropriate dilution factor was determined by pre-experiment Background height Contamination of chromogenic solution Change the color developing solution Color development time is too long Controlling color development time Wrong dilution of detection antibody or enzyme conjugate Use recommended dilution method Incomplete plate washing Sufficient number of washes and addition of sufficient amount of washing liquid Theory This kit uses double antibody sandwich enzyme-linked immunosorbent assay (ELISA)

Background Pepsin is an endopeptidase that breaks down proteins into smaller peptides

Human IL-29 ELISA Kit RNA Pull Down ASM/H12 Immunogen Synthetic Peptide LocationProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20