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Add 100uL detection antibody to each microwell
Epub 2022 May 4
RRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKTSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLIMQLMPFGSLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA
11Add 100ul HRP enzyme labeled anti mouse & Rabbit secondary antibody polymer was incubated for 30 min Note: this liquid is sensitive to light
KIT Strep Tag Protein, Human Proteins and Enzymes Add 100uL detection antibody toProduct Specification Species Human Accession P10721 Amino Acid Sequence T544 V976 Expression System Baculovirus InsectCells Molecular Weight 51. 9 KDa Purity 87% by SDS PAGE Endotoxin <1EU g Conjugation Unconjugated Tags & Cleavage sites N Twin strep Physical Appearance Liquid Storage Buffer 50mM Tris (pH 7. 5), 200mM NaCl, 5% glycerol, , 1 mM DTT. Stability & Storage 80 Guidelines Please use rapid thawing with running water to thaw the protein
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