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immortal cells or tumor cells
then gently mix (concentration is 800 mIU/mL)
where mutations in the SOD1 gene can lead to protein misfolding and aggregation
4 mM DTT should be added when using buffers that have been stored for long periods of time or buffers that have been repeatedly freeze-thawed
Human MIF ELISA Kit Tag Antibody immortal cells or tumor cellsProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
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